Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals

Authors:	Alexis Aspe Alejandra Orrego Emilio Alarcn Camilo Lpez-Alarcn Horacio Poblete Danilo Gonzlez-Nilo

Institution:	^aFacultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 Correo 33, Santiago, Chile;^bDepartamento de Farmacia, Facultad de Química, Pontificia Universidad Católica de Chile, Santiago, Chile;^cCenter for Bioinformatics and Molecular Simulations, Universidad de Talca, Talca, Chile

Abstract:	A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Binding association constants for the nitroxide probes C₃₄₃T and QT with HSA were 5 × 10⁴ and 9 × 10⁴ M⁻¹, respectively. Rate constants for the nitroxide reduction by antioxidants in HSA were determined finding k_HSA/k_buffer ratio of 0.8, 1.9, and 0.075 for ascorbic acid, Trolox, and caffeic acid, respectively, for the nitroxide C₃₄₃T reduction.

Keywords:	Nitroxide prefluorescent probes Antioxidants Nitroxide reduction Human serum albumin Binding constant
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