Ufficio Sicurezza e Prevenzione del CNR, Corso Stati Uniti 4, 35020 Padova, Italy
Istituto di Fotochimica e Radiazioni d'Alta Energia del CNR, Sezione di Legnaro, Via Romea 4, 35020 Legnaro Padova, Italy
Abstract:
A new method of physically immobilizing enzymes in poly(2-hydroxyethyl methacrylate) membranes was developed in order to obtain suitable biosensors. It was possible to prepare an enzyme sensor based on an oxygen Clark electrode and on glucose oxidase immobilized by low-temperature gamma radiation-induced polymerization. Temperature and pH effects on the activity of immobilized enzyme are described and the response characteristics of the resulting biosensor are summarized. The determination of glucose in standard solutions was carried out and a linear calibration curve, with an R2 value of 0·9993, from the detection limit 5 × 10?5
to 1·2 × 10?3
was obtained. The biosensor was employed to analysis of control sera and the results were compared to those obtained by enzymatic-spectrophotometric detection.