HP1/ORC complex and heterochromatin assembly |
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Authors: | Shareef Mohammed M Badugu RamaKrishna Kellum Rebecca |
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Institution: | (1) School of Biological Sciences, University of Kentucky, 101 T.H. Morgan Building, Lexington, KY 40506, USA |
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Abstract: | We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylated isoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed. |
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Keywords: | heterochromatin assembly heterochromatin protein 1 HP1/ORC-associated protein methyltransferase origin recognition complex Su(var)3-9 |
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