Glucocorticoid receptors in subpopulations of human lymphocytes defined by monoclonal antibodies |
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| Authors: | V R Martins M I Birolli A J Duarte M M Brentani |
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| Institution: | 1. RWTH Aachen University, Dept. of Rheumatology, Germany;2. RWTH Aachen University, Translational Pediatric Rheumatology and Immunology, Germany;3. Department of Paediatrics, Inselspital University of Bern, Pediatric Rheumatology, Switzerland;1. Department of Rheumatology, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo 693-8501, Japan;2. Integrated Kidney Research and Advance, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo 693-8501, Japan |
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| Abstract: | Glucocorticoid receptors (GR) were investigated in subpopulations of lymphocytes identified by monoclonal antibodies. Purified T (OKT3+) and non-T lymphocyte subpopulations were isolated from human peripheral blood using Degalan bead columns coated with rabbit anti-human IgG. Purified subpopulations of OKT4+ and OKT8+ lymphocytes were obtained by coating the nonadherent population (T cells) from the first column with OKT4+ or OKT8+ and pouring it into a second Degalan column, coated with goat anti-mouse IgG. GR content and affinity were analyzed by a whole cell assay with 3H]dexamethasone as tracer. The numbers of GR in lymphocyte subpopulations (OKT3+ cells, non-T cells, OKT4+, and OKT8+ cells) were nearly equal. It is concluded that the differential effects of glucocorticoids on the circulatory kinetics of OKT4+ and OKT8+ cells probably are not related to differences in glucocorticoid receptors of these T-cell subpopulations. |
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