Cell of the mucous membrane of the female genital tract in culture: A comparative study with regard to the histogenesis of endometriosis |
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Authors: | Gertrude Mungyer Wim N P Willemsen Rune Rolland Hans M Vemer Francs C S Ramaekers Paul H K Jap Lambert G Poels |
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Institution: | (1) Department of Cytology and Histology, University of Nijmegen, Geert Grooteplein Noord 21, 6500 HB Nijmegen, Th Netherlands;(2) Department of Obstetrics and Gynecology, University of Nijmegen, Geert Grooteplein Noord 21, 6500 HB Nijmegen, Th Netherlands;(3) Department of Pathologic Anatomy, University of Nijmegen, Geert Grooteplein Noord 21, 6500 HB Nijmegen, Th Netherlands |
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Abstract: | Summary Cellular elements from the mucous membrane of the uterus and oviducts and from peritoneal washings were cultured. The in vitro
behavior of these cells was compared to elucidate the histogenesis of endometriosis and the role of various diagnostic procedures.
In 65% of the cultured matrial obtained by uterine-tubal flushing, proliferating cells of the uterine-tubal mucous membrane
were present. Their morphology and behavior corresponded to those of cultured cells obtained by separate washing of the uterine
cavity and the tubes, respectively, curetted material, and biopsies of endometriosis lesions.
Epithelial and stromal cells were identified using phase contrast microscopy, electron microscopy, and immunohistochemical
methods. These cell types did not occur in peritoneal washings before the flushing of uterus and tubes. It was therefore assumed
that they were detached and transported to the pelvic cavity during the above-mentioned procedures. In view of their intensive
proliferation they may form the basis in the development of nodules of endometriosis. This would support the implantation
theory concerning the pathogenesis of endometriosis. Interactions between epithelial and mesothelial cells point to the possible
role of the latter in encapsulating the endometrial elements. |
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Keywords: | endometrium endometriosis tissue culture ultrastructure intermediate filaments |
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