多头绒泡菌间期细胞核和中期染色体中的银染蛋白分析*

电镜原位观察结合图象分析研究了多头绒泡菌Physarum polycephalum Schw间期细胞核和中期染色体中银染蛋白的形状、大小和分布。结果看到,银染蛋白主要呈颗粒状存在于间期细胞核和中期染色体中。银粒的大小不一,分布不均匀。间期细胞核中存在众多直径在5~15nm的银粒,其中10nm以上的较大银粒主要分布于核仁,集缩染色质和核基质部分10nm以上银粒不多。中期细胞核内10nm以上的较大银粒主要分布于染色体中。染色体中除含有一些较大银粒外,多数银粒的直径为5~10nm。本文结果提示,构成染色体骨架的嗜银蛋白可能来自间期细胞核的染色质、核基质和核仁。

ANALYSIS OF SILVER-STAINED PROTEINS IN INTERPHASE NUCLEI AND METAPHASE CHROMOSOMES OF PHYSARUM POLYCEPHALUM

The shape,size and scatter distribution of silver-stained proteins in interphase nuclei and metaphase chromosomes of Physarum polycephalum were studied by observation with electronmicroscopy in situ and image analysis. It was observed that silver-stained proteins existed in interphase nuclei and metaphase chromosomes mainly in the form of granules. The silver granules was not same in size and unequally distributed. In interphase nuclei there were many silver granules with the diameter of 5~15 nm. Among them the silver granules over 10 nm were mainly distributed in nucleoli, and there were few silver granules over 10 nm in condensed chromatin and nuclear matrix. The silver granules over 10 nm in metaphase nuclei were mainly distributed in chromosomes. Except for the bigger ones, the diameter of most silver granules were 5~10 nm. The result of this paper suggeste that silver-stained chromosome scaffold proteins may come from the condensed chromatin, nuclear matrix and nucleolus of interphase nucleus.