|
|||||
|
|
A fluorimetric method for quantitation in the picomole range of N1-methylnicotinamide and nicotinamide in serum. |
|
| Authors: | B R Clark R M Halpern R A Smith |
| Institution: | 1. Department of Psychiatry, University of California, Los Angeles, California 90024 USA;2. Department of Medicine, University of California, Los Angeles, California 90024 USA;3. the Molecular Biology Institute, University of California, Los Angeles, California 90024 USA;4. Department of Chemistry, University of California, Los Angeles, California 90024 USA |
| Abstract: | A method is described for the fluorimetric determination of N1-methylnicotinamide in deproteinized serum extract and of nicotinamide after extraction into ethyl acetate from deproteinized serum extract and subsequent conversion to N1-methylnicotinamide. N1-methylnicotinamide is converted to fluorescent derivatives by treatment with acetophenone in alcoholic KOH followed by addition of 99% formic acid. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |