Utility of gel-free,label-free shotgun proteomics approaches to investigate microorganisms |
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Authors: | Bianca Porteus Chawalit Kocharunchitt Rolf E Nilsson Tom Ross John P Bowman |
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Institution: | (1) Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, College Road, Sandy Bay, 7005 Hobart, Tasmania, Australia; |
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Abstract: | This review will examine the current situation with label-free, quantitative, shotgun-oriented proteomics technology and discuss
the advantages and limitations associated with its capability in capturing and quantifying large portions of proteomes of
microorganisms. Such an approach allows (1) comparisons between physiological or genetic states of organisms at the protein
level, (2) ‘painting’ of proteomic data onto genome data-based metabolic maps, (3) enhancement of the utility of genomic data
and finally (4) surveying of non-genome sequenced microorganisms by taking advantage of available inferred protein data in
order to gain new insights into strain-dependent metabolic or physiological capacities. The technology essentially is a powerful
addition to systems biology with a capacity to be used to ask hypothesis-driven ‘top-down’ questions or for more empirical
‘bottom-up’ exploration. |
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