Predominance of type III over type I cell surface procollagens in rat lung fibroblasts |
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| Authors: | Y Nagai |
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| Institution: | Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405. |
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| Abstract: | Binding assays of 125I-labeled affinity-purified monospecific type I and III procollagen antibodies to fibroblasts have revealed the presence of type I and III procollagens on the cell surface, and that the amount of type III procollagen is 10 times greater than that of type I procollagen. Analyses by flow cytometry and fluorescent microscopy after treatment with an isothiocyanate (FITC)-conjugated second antibody supported this finding. |
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