Specific antibody-dependent activation of neutrophils by liposomes containing spin-label lipid haptens. |
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| Authors: | D G Hafeman J W Parce H M McConnell |
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| Affiliation: | Stauffer Laboratory for Physical Chemistry Stanford University, Stanford, California 94305 USA |
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| Abstract: | We have found that superoxide production by human neutrophils can be stimulated by liposomal membranes containing nitroxide spin-labeled lipids in the presence of specific rabbit antibodies directed against the nitroxide group. The extent of superoxide production was found to depend strongly on lipid composition under conditions where the concentration of antibodies and number of exposed haptens were maintained constant. The dependence of neutrophil activation on the physical properties of the liposomal lipid membrane appears to be similar to the dependence of complement depletion on these physical properties. |
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| Keywords: | DPPC dipalmitoylphosphatidylcholine DMPC dimyristoylphosphatidylcholine egg PC egg phosphatidylcholine chol cholesterol HEPES N-2-hydroxyethylpiperazine-N′-2-ethansulfonic acid |
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