共价连接的多肽酶联免疫测定法的研究

采用紫外线照射及戊二醛活化处理聚苯乙烯酶标板后再包被八肽胆囊收缩素（CCK-8），以此为基础进行酶联免疫吸附试验（ELISA），通过不同的洗涤过程对CCK-8与酶标板相互作用的性质进行研究.结果表明，该包被方法可使CCK-8通过共价交联的方式稳定地结合于固相载体上，该方法适用于不同来源的酶标板，处理后的酶标板性质稳定，可储存使用，测定的批内及批间变异系数分别为4.75%和7.80%，方法稳定可靠，重复性好，便于推广应用.

Study on Covalent-attached Enzyme-linked Immunoassay for Polypeptide

In order to study the mechanism of enzyme linked immunoassay for octapeptide cholecystokinin(CCK 8),ELISA procedures were based on the pretreatment that polystyrene microplates were irradiated by ultraviolet and activated by glutaraldehyde prior to coating,and the binding nature of CCK 8 to microplates was revealed by different washing methods including dH 2O,PBS T and SDS. The results showed that this pretreatment led to the covalent attachment of CCK 8 to the solid phase steadily and fit to different sources of microplates. The treated microplates could be stored at least 4 weeks not to influence their ability of binding antigens.The intra and inter variation coefficients were 4 75% and 7 80% respectively.This polypeptide ELISA was characterized by covalent attachment between antigen and solid phase carrier and was proved to be highly stable,reproducable and available.