Relation between nuclear envelope and nuclear lamina in nuclear assembly in vitro |
| |
Authors: | CAI Shutao ZHAI Zhonghe |
| |
Affiliation: | (1) Life Sciences College of Peking University, 100871 Beijing, China;(2) Present address: Institute of Biophysics, The Chinese Academy of Sciences, 100101 Beijing, China |
| |
Abstract: | Xenopus laevis egg extracts cell-free nuclear assembly system was used as an experimental model to study the process of nuclear lamina assembly in nuclear reconstitutionin vitro. The experimental results showed that lamin was involved in the nuclear assemblyin vitro. The assembly of nuclear lamina was preceded by the assembly of nuclear matrix, and probably, inner nuclear matrix assembly provided the basis for nuclear lamina assembly. Inhibition of normal assembly of nuclear Iknina, by preincubating egg extracts cell-free system with anti-lamin antibodies, resulted in abnormal assembly of nuclear envelope, suggesting that nuclear envelope assembly is closely associated with nuclear lamina assembly. Project supported by the National Natural Science Foundation of China. |
| |
Keywords: | nuclear assemblyin vitro nuclear envelope nuclear lamina nuclear matrix |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学:生命科学英文版》浏览原始摘要信息 |
|
点击此处可从《中国科学:生命科学英文版》下载全文 |
|