Characterization of poly(a)-protein complexes isolated from free and membrane-bound polyribosomes of Ehrlich ascites tumor cells |
| |
Authors: | Dick B Janssen Anda D Counotte-Potman Walther J Van Venrooij |
| |
Institution: | (1) Dept. of Biochemistry, University of Nijmegen, Geert Grooteplein Noord 21, Nijmegen, The Netherlands |
| |
Abstract: | Proteins present in messenger ribonucleoprotein particles were labeled with 35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and affinity chromatography on oligo(dT)-cellulose. Both classes of Poly(A)-protein particles contain a poly(A) chain of about 70 adenyl residues and a protein with a molecular weight of 76000 attached to it. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |