Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production |
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Authors: | Ronaldo Z. Mendonça Katia N. Greco Alvaro P. B. Sousa Roberto H. P. Moraes Renato M. Astray Carlos A. Pereira |
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Affiliation: | 1.Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brasil 1500, 05503-900 São Paulo, Brasil ;2.Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brasil |
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Abstract: | Gene expression in animal cells allows large scale production of proteins used for either structure and function studies or therapeutic purposes. Maximizing recombinant protein production is necessary to optimize cell growth and protein expression. Some studies have demonstrated the presence of pharmacologically active substances in insect hemolymph. In this work, we have identified and purified a protein from Lonomia obliqua hemolymph able to increase the production of the rabies virus glycoprotein, expressed in Drosophila melanogaster S2 cells, by about 59%. |
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Keywords: | Insect cells Recombinant Lonomia obliqua Purification Rabies Hemolymph |
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