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A colorimetric method for the estimation of phloroglucinol
Authors:N P Jayasankar  J V Bhat
Affiliation:1. Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India;2. Departments of Chemistry and Chemical Engineering, Indian Institute of Technology Jammu, Jammu & Kashmir 181221, India;1. Department of Ophthalmology, Seoul National University College of Medicine, Seoul, South Korea;2. Department of Ophthalmology, Seoul National University Hospital, Seoul, South Korea;3. Kong Eye Hospital, Seoul, South Korea;4. Department of Ophthalmology, Seoul National University Boramae Hospital, Seoul, South Korea;5. Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, South Korea;6. Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea;1. School of Maritime Economics and Management, Dalian Maritime University, Dalian 116026, China;2. Department of Business Administration, National Taiwan University, No.1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC;1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China;2. Department of Bioinformatics and Biological Statistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China;3. State Key Laboratory of Microbial Metabolism, Shanghai-Islamabad-Belgrade Joint Innovation Center on Antibacterial Resistances, Joint Laboratory of International Cooperation in Metabolic and Developmental Sciences, Ministry of Education and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, PR China;4. Peng Cheng Laboratory, Vanke Cloud City Phase I Building 8, Xili Street, Nashan District, Shenzhen, Guangdong, 518055, PR China;5. Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, PR China
Abstract:
  • 1.1. A simple method has been devised for the estimation of phloroglucinol based on the formation of an intense colored compound with a modified Ehrlich reagent in the presence of trichloroacetic acid. Some factors affecting the formation of color have been studied.
  • 2.2. Careful regulation of trichloroacetic acid content in the system permits its estimation in 1–15 μg in the micro range and in 10–50 μg in the macro range. Phloroglucinol lends itself to ready separation by paper chromatography and estimation after clution from paper.
Keywords:
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