Effect of Boron on Osteogenic Differentiation of Human Bone Marrow Stromal Cells |
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Authors: | Xiaozhou Ying Shaowen Cheng Wei Wang Zhongqin Lin Qingyu Chen Wei Zhang Dongquan Kou Yue Shen Xiaojie Cheng Ferdinand An Rompis Lei Peng Chuan zhu Lu |
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Affiliation: | Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China. |
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Abstract: | Bone marrow stromal cells (BMSCs) have been well established as an ideal source of cell-based therapy for bone tissue engineering applications. Boron (B) is a notable trace element in humans; so far, the effects of boron on the osteogenic differentiation of BMSCs have not been reported. The aim of this study was to evaluate the effects of boron (0, 1, 10,100, and 1,000?ng/ml) on osteogenic differentiation of human BMSCs. In this study, BMSCs proliferation was analyzed by cell counting kit-8 (CCK8) assay, and cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, Von Kossa staining, and real-time PCR. The results indicated that the proliferation of BMSCs was no different from the control group when added with B at the concentration of 1, 10, and 100?ng/ml respectively (P?>?0.05); in contrast, 1,000?ng/ml B inhibited the proliferation of BMSCs at days?4, 7, and 14 (P?0.05). By ALP staining, we discovered that BMSCs treated with 10 and 100?ng/ml B presented a higher ALP activity compared with control (P?0.05). By real-time PCR, we detected the messenger RNA expression of ALP, osteocalcin, collagen type I, and bone morphogenetic proteins 7 were also increased in 10 and 100?ng/ml B treatment groups (P?0.05). The calcium depositions were increased in 1 and 10?ng/ml B treatment groups (P?0.05). Taken all together, it was the first time to report that B could increase osteogenic effect by stimulating osteogenic differentiation-related marker gene synthesis during the proliferation and differentiation phase in human BMSCs and could be a promising approach for enhancing osteogenic capacity of cell-based construction in bone tissue engineering. |
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