The relationship of purity to photosynthetic activity in preparations ofCodium fragile chloroplasts

Summary The purity of the chloroplasts isolated from the algaCodium fragile by 1. a conventional centrifugation method, and 2. a method involving a gel filtration stage through Sephadex G 50 coarse, was compared using enzymatic analysis in conjunction with electron microscopy. The findings show that the purer chloroplasts, obtained by gel filtration, could carboxylate more¹⁴CO₂ and evolve more O₂ than the crude preparation from which they originated.