Identification and characterization of a novel prolyl oligopeptidase in filarial parasite Setaria cervi |
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| Authors: | Mohit Wadhawan Savitri Tiwari Shweta Sharma Sushma Rathaur |
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| Institution: | Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, India |
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| Abstract: | A 75 kDa serine protease having prolyl oligopeptidase activity has been purified from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 6 peptides showing nearest match S9A (prolyl oligopeptidase) family protein from Plesiocystis pacifica. The ScPOP was found to be unique compared to mammalian POP with respect to its kinetic properties. To elucidate its role, filarial parasites were exposed to specific inhibitor of POP, Z-Pro-prolinal (ZPP) for 8?h. The inhibition of POP induced calcium signaling via phospholipase c stimulation which further triggered mitochondrial mediated apoptosis in filarial parasites. |
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| Keywords: | Prolyl oligopeptidase ER stress Calcium Mitochondria Apoptosis Parasite |
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