Biomineralization Consolidation of Fresco Wall Paintings Samples by Bacillus sphaericus |
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Authors: | Fatma M. Helmi Hemdan R. Elmitwalli Abeer F. El-hagrassy |
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Affiliation: | 1. Conservation Department, Faculty of Archaeology, Cairo University, Giza, Egypt;2. Conservation Department, Faculty of Archaeology, Fayoum University, Fayoum, Egypt |
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Abstract: | Bacterial-induced mineralization has been explored for protection and consolidation of degraded limestone, concrete and plaster by precipitation of calcium carbonate. It is the first time that Bacillus sphaericus was used for consolidating the nonsterilized decayed wall paintings samples by immersing them in sterile nutritional media. The B. sphaericus used in this study produced urease, which catalyzes the hydrolysis of urea (CO(NH2)2) into ammonium (NH4) and carbonate (CO3?2) leading to the precipitation of calcium carbonate. The effect of B. sphaericus on wall paintings was determined by recording the evolution of culture media chemistry and examining the treated wall paintings under a scanning electron microscope to show the structural and morphological evolution of calcium carbonate that was investigated in wall paintings models. |
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Keywords: | Bacillus sphaericus bioconsolidation biomineralization calcium carbonate fresco wall paintings ureolytic bacteria |
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