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Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes |
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| Authors: | M Moeremans G Daneels J De Mey |
| Affiliation: | 1. Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran;2. Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;3. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;4. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran |
| Abstract: | A sensitive staining method for protein blots on nitrocellulose membranes is described and compared with commonly used dye staining methods. It uses colloidal metal sols (gold or silver) stabilized with Tween 20 and adjusted to pH 3. It is based on the selective high-affinity binding of colloidal metal particles to the proteins and produces a red-purplish color (gold) or dark grey (silver). The sensitivity of this new staining method is in the same range as silver staining of polyacrylamide gels and matches the sensitivity of overlay assays. It will therefore be a useful tool for correlating the position of bands or spots of proteins detected with overlay assays with the complete electropherogram in a duplicate protein blot. |
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