Fluorescence properties of a benzo(a)pyrene 7,8 dihydrodiol 9,10-oxide-DNA adduct. Conformation and effects of intermolecular DNA interactions期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Fluorescence properties of a benzo(a)pyrene 7,8 dihydrodiol 9,10-oxide-DNA adduct. Conformation and effects of intermolecular DNA interactions

Authors:	T Prusik N E Geacintov

Affiliation:	1. Chemistry Department New York University, New York, New York, 10003, USA;2. Radiation and Solid State Laboratory, New York University, New York, New York, 10003, USA;3. Squibb-Institute for Medical Research New Brunswick, New Jersey 08903 USA

Abstract:	The pyrene-like fluorescence of the covalent benzo(a)pyrene diol-epoxide-DNA complex prepared by reacting 7,8,-dihydrodiol 9,10-epoxy benzo(a)pyrene (BPDE) with DNA in aqueous solution $in vitro$ , has been investigated. It is shown that this fluorescence is $sensitive$ to molecular oxygen, to the concentration of $native$ DNA and to the ionic strength (KCl concentration), but is $insensitive$ to the concentration of $denatured$ DNA. These effects are related to the conformation of the pyrene-like chromophore of BPDE. Most of the fluorescence of a $dilute$ solution of the DNA-bound benzo(a)pyrene derivative originates from binding sites in which the pyrene moiety is not intercalated between the DNA base pairs, but is located on the outside of the DNA double helix.

Keywords:	To whom correspondence should be addressed.
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