Long-term cryopreservation of sperm of Japanese eel |
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| Authors: | S Tanaka H Zhang N Horie Y Yamada A Okamura T Utoh N Mikawa H P Oka H Kurokura |
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| Institution: | IRAGO Institute, Shinden 377-Ehima, Atsumi-cho, Atsumi-gun, Aichi 441-3605, Japan;Tokyo University, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan |
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| Abstract: | After cryopreservation in a diluent (D#5-D), containing 76·2 mM NaHCO3, 137·0 mM NaCl, 1·4% soya lecithin and 10% dimethyl sulphoxide, for 30–180 days, Japanese eel Anguilla japonica sperm was 61·4–70·6% motile compared with fresh sperm. Cryopreserved sperm was used for fertilizing three batches of eggs and hatchability ranged from 20·7 to 69·1%. These results indicate that D#5-D is an useful diluent for sperm cryopreservation in Japanese eel. |
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| Keywords: | Japanese eel sperm cryopreservation sodium bicarbonate hatchability |
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