| 富铬蚁巢伞（鸡菌）液体培养(英文) |
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| 引用本文： | 胡忠策,郑晓冬,陈新爱,郑裕国.富铬蚁巢伞（鸡菌）液体培养(英文)[J].菌物学报,2008,27(1). |
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| 作者姓名： | 胡忠策 郑晓冬 陈新爱 郑裕国 |
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| 摘 要： | A species of mushroom, Termitomyces albuminosus, was cultured in liquid medium for production of chromium-enriched mycelium. The influence of chromium (Ⅲ ) on mycelial growth of T. albuminosus was investigated. An optimum medium composed of 5.6g/L yeast extract, 51.6g/L hydrolyzed rice, 2g/L KH2PO4, and 20mg/L chromium(Ⅲ ) with initial pH of 4.5 was obtained by using method of central composite design (CCD). After incubation of 84h, the maximal biomass of chromium-enriched mycelia reached 24.23g DMW(dried mycelial weight)/L with 272μg/g DMW chromium content in 500mL flasks containing 100mL medium with an inoculum of 8% on a shaker of 100r/min under an optimized cultivation condition at 28℃.
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Submerged culture of chromium-enriched Termitomyces albuminosus |
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| Authors: | HU Zhong-Ce ZHENG Xiao-Dong CHEN Xin-Ai ZHENG Yu-Guo |
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| Abstract: | A species of mushroom, Termitomyces albuminosus, was cultured in liquid medium for production of chromium-enriched mycelium. The influence of chromium (Ⅲ) on mycelial growth of T. albuminosus was investigated. An optimum medium composed of 5.6g/L yeast extract, 51.6g/L hydrolyzed rice, 2g/L KH2PO4,and 20mg/L chromium(Ⅲ) with initial pH of 4.5 was obtained by using method of central composite design (CCD). After incubation of 84h, the maximal biomass of chromium-enriched mycelia reached 24.23g DMW(dried mycelial weight)/L with 272μg/g DMW chromium content in 500mL flasks containing 100mL medium with an inoculum of 8% on a shaker of 100r/min under an optimized cultivation condition at 28℃. |
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| Keywords: | glucose tolerance factor medium optimization growth kinetics |
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