利用生物化学标记分析鉴定一个抗小麦黄矮病的小麦新种质

利用多个生化标记系统对一个抗小麦黄矮病新种质（ＨＧ２９５）进行了分析鉴定，胚乳水溶蛋白等电聚焦表明，在ｐＨ８．０－１０．２范围内，中５有３条在其小麦亲本南大２４１９、克强中匀不存在的克异带，其中只有和经２条在ＨＧ２９５中得到表达。在ＨＧ２９５中，２ＤＳ控制的那条水溶蛋白带发生了的缺失，ＨＧ２９５中２ＤＳ的缺失在Ｐｅｒ－５与Ｅｓｔ－６电泳分析中进一步得到确证，Ｅｓｔ－７酶谱表明，中５与ＨＧ２９５均表

Characterization of a Wheat Germplasm for BYDV Resistance Using Biochemical Markers

Several biochemical marker systems were used to characterize a BYDV resistant wheat germplasm, HG295. IEF phenotype of water soluble protein from endosperm indicated that Zhong 5 expressed three novel bands in the pH range 8.0-10.2, the second of them was also expressed in HG295. Moreover, the 2DS WSP band was missing in HG295. The absence of 2DS in HG295 was also confirmed by Per-5 and Est-6 analysis. The phenotype of Est-7 indicated that the 2DL was possibly missingin HG295. The absence of 2DL was further confirmed by Superoxide dismutase (SOD-1) phenotype of HG295, Moreover, both Zhong 5 and HG295 expressed a novel SOD-1 band other than their wheat background. Various results provided convincing evidences that HG295 is a 2E (X)/2D substitution line.