PCR对ES细胞定点整合重组子的鉴定

ＰＣＲ是一种对ＥＳ细胞定点整合重组子进行鉴定的有效方法。由于在定点整合重组子和非定点整合重组子中外源导入基因与基因组ＤＮＡ分子整合的方式各不相同。因此，非重组子、定点整合重组子和非定点整合重组子的基因ＤＮＡ结构也将各不相同。因此，非重组子、定点整合重组子和非定点整合重组子的基因组ＤＮＡ分子结构也将各不相同。当我们设计合适的引物，从ＰＣＲ扩增结果中即可分析、鉴别出定点整合重组子、非定点整合重组子、或

PCR Detecting Homologous Recombinants from ES Cell

PCR is an effective method for detecting homologous recombinants from ES cells,because there are different genomic DNA structure among non-recombinants,site specific recombinants and non-site specific recombinants.When we design suitable primers,we can analyze and identify site specific recombinants,non-site specific recombinants and non-recombinants.In this paper,we design two different pairs of primer(according to the genomic DNA patterns) for using PCR to detect the cells which resist to G418 and 6-TG after plasmid pRV4.0 transforming male mouse ES cells.we get the homologous recombinants quickly,simply and specifically.We test the reliability of PCR using southern blot hybridization.