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棉花纤维发生相关AFLP标记
引用本文:罗明,李名扬,侯磊,肖月华,张正圣,裴炎,季道藩. 棉花纤维发生相关AFLP标记[J]. 遗传学报, 2001, 28(7): 677-682
作者姓名:罗明  李名扬  侯磊  肖月华  张正圣  裴炎  季道藩
作者单位:1. 西南农业大学生物技术中心,
2. 浙江大学,
基金项目:国家自然科学基金重点资助项目(批准号:39830240);国家教委博士点基金(批准号:1999-006003)
摘    要:以徐州142及其无绒无絮(无纤维)突变体(fl)为材料,利用AFLP标记技术对该近等基因系进行DNA多态性分析。在64对引物产生的6360多条扩增带中,发现1条差异带(CF1)稳定出现在有纤维和无纤维之间。进一步用徐州142无绒无絮突变系与纤维发育正常的渝棉一号的杂交后代F2、F3分离群体以及几个陆地棉品种作材料验证。结果表明,差异带(CF1)与纤维的产生呈共分离,该差异带是与棉花纤维发生相关的分子标记。进而对CF1进行回收、克隆和序列测定,CF1长205bp,通过6个开放阅读框推断的氨基酸序列,与酚羟化酶α-亚基、外表皮蛋白C、NADH脱氢酶亚基1、NADH-CoQ氧化还原酶、2-氧代-铁氧化蛋白-氧化还原酶以及假拟14.5kD蛋白(hypotheitcal 14.5kD protein)有较高的相似性。

关 键 词:棉花发育 纤维发育 AFLP 分子标记 相关性
文章编号:0379-4172(2001)07-0677-06
修稿时间:2000-12-06

An AFLP Marker Related to Fibrogenesis in Upland Cotton
LUO Ming,LI Ming-Nang,HOU Lei,XIAO Yue-Hua,ZHANG Zheng-Sheng,PEI Yan,JI Dao-Fan. An AFLP Marker Related to Fibrogenesis in Upland Cotton[J]. Journal of Genetics and Genomics, 2001, 28(7): 677-682
Authors:LUO Ming  LI Ming-Nang  HOU Lei  XIAO Yue-Hua  ZHANG Zheng-Sheng  PEI Yan  JI Dao-Fan
Abstract:By using AFLP technique polymorphism analysis was performed between a fuzzlesslintless mutant line and its isogenic wild-type line, Xuzhou 142. Out of 6 360 bands produced by 64 pairs primers, a fragment named as CF1, appearing stably in wild-type line, Xuzhou 142. This polymorphism was further verified using several normal fiber varieties and F2, F3 populations from the cross of fuzzless-lintless mutant line with a high-lint-percentage variety Yumian No. 1. The cosegregation of CF1 and fibrogenesis was proved, which suggested that CF1 can be used as a molecular marker for cotton fibrogenesis. The CF1 segment was cloned into PUCm-T Vector and then sequenced. The putative amino acid sequences, is an analogue to phenol hydroxylase alpha subunit outer surface protein C, NADH dehydrogenase subunit 1, NADH- ubiquinone oxidoreductase, 2-oxoacid ferredoxin oxidoreduct and hypothetical 14.5kD protein.
Keywords:upland cotton  fiber  AFLP  marker
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