| 一株琥珀酸产生菌的筛选及鉴定 |
| |
| 引用本文： | 朱蕾蕾,刘宇鹏,郑璞,孙志浩.一株琥珀酸产生菌的筛选及鉴定[J].微生物学通报,2007,34(1):0080-0084. |
| |
| 作者姓名： | 朱蕾蕾 刘宇鹏 郑璞 孙志浩 |
| |
| 作者单位： | 江南大学生物催化研究室江南大学工业生物技术教育部重点实验室,无锡,214036 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);江苏省自然科学基金;教育部长江学者和创新团队发展计划 |
| |
| 摘 要： | 从牛的瘤胃中筛选获得一株能发酵生产琥珀酸的兼性厌氧菌。对其进行生理生化特性鉴定及16S rRNA基因分析。该菌株短杆状,无鞭毛,革兰氏染色阴性,V-P反应阴性,能发酵多种糖类产酸;其16S rRNA基因与琥珀酸放线杆菌的同源性高达99．8%,认为属于琥珀酸放线杆菌(Actinobacillus succinogenes),并将其命名为琥珀酸放线杆菌(Actinobacillus succinogenes)SW0580,保藏号CGMCC 1593。初步发酵试验表明该菌能发酵60g/L葡萄糖产生25．8g/L的丁二酸。
|
| 关 键 词： | 琥珀酸放线杆菌 琥珀酸 分离筛选 鉴定 厌氧发酵 |
| 文章编号： | 0253-2654（2007）01-0080-05 |
| 修稿时间： | 2006-04-12 |
Screening and Identification of a Strain of Actinobacillus succinogenes Producing Succinic Acid by Anaerobic Fermentation |
| |
| ZHU Lei-Lei,LIU Yu-Peng,ZHENG Pu and SUN Zhi-Hao.Screening and Identification of a Strain of Actinobacillus succinogenes Producing Succinic Acid by Anaerobic Fermentation[J].Microbiology,2007,34(1):0080-0084. |
| |
| Authors: | ZHU Lei-Lei LIU Yu-Peng ZHENG Pu and SUN Zhi-Hao |
| |
| Institution: | Laboratory of Biocatalysis; School of Biotechnology; Southern Yangtze University; The Key Laboratory of Industrial Biotechnology; Ministry of Education; Wuxi 214036;Laboratory of Biocatalysis; School of Biotechnology; Southern Yangtze University; The Key Laboratory of Industrial Biotechnology; Ministry of Education; Wuxi 214036;Laboratory of Biocatalysis; School of Biotechnology; Southern Yangtze University; The Key Laboratory of Industrial Biotechnology; Ministry of Education; Wuxi 214036;Laboratory of Biocatalysis; School of Biotechnology; Southern Yangtze University; The Key Laboratory of Industrial Biotechnology; Ministry of Education; Wuxi 214036 |
| |
| Abstract: | A strain,Actinobacillus succinogenes CGMCC 1593,producing succinic acid was isolated from bovin rumen,which could pro- duces succinic acid by anaerobic fermentation.A series of morphological and biochemical characteristics and sequence analysis of 16S rDNA reveal that it belongs to Actinobacillus succinogenes.When cultured anaerobically in medium containing 60 g/L glucose as carbon source,the strain produces 25.8 g/L of succnic acid. |
| |
| Keywords: | Actinobacillus succinogenes Succinic acid Screening Identification Anaerobic fermentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
