Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines |
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Authors: | Neil Sidell Adrienne Altman Mark R. Haussler Robert C. Seeger |
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Affiliation: | 1. Division of Surgical Oncology, Department of Pediatrics, UCLA School of Medicine, Los Angeles, CA 90024 USA;2. Division of Cancer Immunology and Biology, Department of Pediatrics, UCLA School of Medicine, Los Angeles, CA 90024 USA;3. Department of Biochemistry, University of Arizona Health Sciences Center, Tucson, AZ 85724, USA |
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Abstract: | It has been shown that retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. The present study tests the histological generality of these phenomena by determining the effects of RA on seven other human neuroblastoma cell lines. Results show that RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in six of seven of the cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in four of the lines, cellular enlargement and vacuolization in one culture, and formation of large, flattened epithelial or fibroblastic-like cells in another culture. Although one cell line was relatively insensitive to the effects of RA in monolayer culture, all seven were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. These findings suggest that, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer. |
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Keywords: | To whom offprint requests should be sent. Address: Division of Surgical Oncology UCLA School of Medicine. University of California Los Angeles CA 90024 USA. |
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