Progress through G1 and S in relation to net protein accumulation in human NHIK 3025 cells |
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Authors: | Øystein W. Rønning Tore Lindmo |
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Affiliation: | Departments of Tissue Culture and Biophysics, Norsk Hydro''s Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo 3, Norway |
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Abstract: | We have investigated whether human NHIK 3025 cells are dependent upon a net increase in cellular protein content in order to traverse G1 and S. The increase in DNA and protein content was studied by means of two-parameter flow cytometry using populations of cells synchronized by mitotic selection. By adding 1 μM cycloheximide to the medium protein synthesis was partially inhibited, resulting in negligible net accumulation of protein. The cells were able to enter S and progress through S under such conditions. The latter was the case whether the cells had been accumulating protein during G1 or not. The results further indicate that the larger cells enter S earlier and traverse S at a higher rate than the smaller cells. Our conclusion is that net accumulation of protein does not seem to be a prerequisite for traverse through G1 and S, i.e. DNA replication may be dissociated from the general growth of cell mass. |
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