Fast screening method for detection of acyl-HSL-degrading soil isolates |
| |
Authors: | Jafra Sylwia van der Wolf Jean Martin |
| |
Institution: | Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. jafra@biotech.univ.gda.pl |
| |
Abstract: | A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc)). |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|