Irradiation of synchronized cell cultures |
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Authors: | H. Firket |
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Affiliation: | (1) Laboratoire d'Anatomie Pathologique, Université de Liège, Belgique |
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Abstract: | Summary When HeLa cells are synchronized by the double thymidine block method, it is shown that the mitotic delay produced by 450 R X-rays is longer when cells are irradiated in G2 than in G1 but longer still when they are irradiated in the early part of S. DNA synthesis, measured by3H-Thymidine incorporation, is not reduced considerably (± 25%) and only for a few hours when 600 R are given at this time. A more pronounced inhibition occurs when irradiation is given later in S. There is also no decrease in incorporation of3H-orotic acid in RNA when the cells are irradiated in S or in G2. These results are discussed and it is concluded that the long mitotic delays cannot he due to a modification of nucleic acids metabolism.Paper read at the 6th Annual Meeting of the European Society for Radiobiology, Interlaken, 5.–8. June, 1968. Round Table: Radiation Effectsin vitro andin vivo. Correlations and Discrepancies. |
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