Production of androsta-1,4-diene-3,17-dione from cholesterol using two-step microbial transformation |
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Authors: | Chung-Yi Lee Ci-Di Chen Wen-Hsiung Liu |
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Institution: | (1) Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China;(2) Present address: Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China |
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Abstract: | A novel two-step transformation process for the production of androsta-l by microorganisms-diene-3,17-dione (ADD) from a high concetration of cholesterol by microorganisms is proposed. Cholesterol (20 g/l) was initially converted to cholest-4-en-3-one (cholestenone) by an inducible cholesterol oxidase-producing bacterium, Arthrobacter simplex U-S-A-18. The maximum productivity of cholestenone was 8 g/l per day and the molar conversion rate was 80%. Subsequently, a fine suspension of cholestenone (50 g/l), which was prepared directly from the fermentation broth of A. simplex, was converted to ADD by Mycobacterium sp. NRRL B-3683 in the presence of an androstenone adsorbent, Amberlite XAD-7. The maximum productivity of ADD was 0.91 g/l per day and the molar conversion rate was 35%.
Correspondence to: W.-H. Liu |
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