Biological activities of Zn(II)-S-methyl-cysteine complex as antiradical,inhibitor of acid phosphatase enzyme and in vivo antidepressant effects |
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| Authors: | Graciela E Escudero Nancy Martini Khalil Jori Nadir Jori Nahuel R Maresca Carlos H Laino |
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| Institution: | 1. Instituto de Bioquímica y Farmacia del IByF-CENIIT-UNLaR (Centro de Investigación e Innovación Tecnológica), Av Luis Vernet y Apostol Felipe, La Rioja, Argentina,;2. Centro de Química Inorgánica (CEQUINOR/CONICET, UNLP), FCE-UNLP, La Plata, Argentina, and;3. Instituto de Biotecnología del CENIIT-UNLaR (Centro de Investigación e Innovación Tecnológica), Av Luis Vernet y Apostol Felipe, La Rioja, Argentina |
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| Abstract: | The antidepressant effect of simple Zn(II) salts has been proved in several animal models of depression. In this study, a coordination metal complex of Zn(II) having a sulfur containing ligand is tested as antidepressant for the first time. Forced swimming test method on male Wistar rats shows a decrease in the immobility and an increase in the swimming behavior after treatment with Zn(S-Met)2] (S-Met=S-methyl-l-cysteine) being more effective and remarkable than ZnCl2. The thiobarbituric acid and the pyranine consumption (hydroxyl and peroxyl radicals, respectively) methods were applied to evaluate the antioxidant activity of S-Met and Zn(S-Met)2] showing evidence of attenuation of hydroxyl but not peroxyl radicals activities. UV-vis studies on the inhibition of acid phosphatase enzyme (AcP) demonstrated that S-methyl-l-cysteine did not produce any effect but, in contrast, Zn(S-Met)2] complex behaved as a moderate inhibitor. Finally, bioavailability studies were performed by fluorescence spectroscopy denoting the ability of the albumin to transport the complex. |
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| Keywords: | Antidepressant effect antioxidant activity phosphatase inhibitory effects S-methyl-cysteine Zn(II) complex |
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