Impact of CYP3A5 and MDR-1 gene polymorphisms on the dose and level of tacrolimus among living-donor liver transplanted patients: single center experience |
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| Authors: | Mona Fathy Manal Kamal Abeer Mohy Ahmad Nabil |
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| Institution: | 1. Clinical and Chemical Pathology Department, Cairo University, Cairo, Egypt and muna_fathy@hotmail.com;3. Clinical and Chemical Pathology Department, Cairo University, Cairo, Egypt and;4. Tropical Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt |
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| Abstract: | Aim of work: To assess the impact of Cytochrome P450 3A5 (CYP3A5) and multidrug resistance-1 gene (MDR-1) single nucleotide polymorphisms on the dose and blood level of tacrolimus among liver transplanted patients.Patients and methods: We enrolled a prospective study of 41 liver transplanted patients. Dose-adjusted trough blood concentration (C/D ratio) was calculated. Polymerase chain reaction-restriction fragment length polymorphism followed by sequencing was done for genotyping of CYP3A5*3 (6986A?>?G).Results: At 1 week, 1 and 3 months C/D ratio were significantly lower in CYP3A5 expressers *1/*1 patients compared to non-expressers *3/*3.Conclusion: CYP3A5 (6986A?>?G) genotype, rather than MDR-1 (2677G?>?A/T) variant, has an impact on tacrolimus pharmacokinetics. |
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| Keywords: | Cytochrome P450 liver transplantation multidrug resistance-1 tacrolimus |
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