Comparative assay of Vipera ammodytes antivenom potency |
| |
| Authors: | Cristian Capitanescu Anca Monica Macovei Oprescu Alexandru Supeanu Bogdan Ioan Coculescu Victor Strambu Radu Alexandru Macovei |
| |
| Institution: | 1. S.C. Ecodet Activ S. R. L, Bucharest, Romania,;2. National Environmental Protection Agency, Bucharest, Romania, cristian.capitanescu@yahoo.com;4. Carol Davila University of Medicine and Pharmacy, Bucharest, Romania,;5. Romanian National Sanitary Veterinary and Food Safety Authority, Bucharest, Romania,;6. University "Titu Maiorescu", Bucharest, Romania, and |
| |
| Abstract: | The finding of the most appropriate way to assess precisely the antivenom efficacy represents one of the major issues for antivenom standardization and success increasing of antivenom therapy. The efficacy of experimental Vipera ammodytes antivenom raised in sheep was determined using in vivo mouse lethality test, respectively, L-aminoacid oxidase, total proteinase and phospholipase A2 antienzymatic effectiveness. The values gained for the antivenom potency depend on the method of measure. So, some of the most toxic venom proteins own phospholipase A2 activity and provide the highest antivenom potency (lowest effective dose) values by antienzymatic assay method. This value is similar with total antiproteolytic antivenom potency value, but almost three times higher than value obtained by L-aminoacid oxidase (low toxic viper venom protein) antienzymatic assay method. |
| |
| Keywords: | Median effective dose L-aminoacid oxidase" target="_blank">L-aminoacid oxidase phospholipase A2 |
|
|
