Gas-chromatographic analysis of poly(3-hydroxyalkanoates) in bacteria |
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| Authors: | Gern N. M. Huijberts Hetty van der Wal Clare Wilkinson Gerrit Eggink |
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| Affiliation: | (1) Agrotechnological Research Institute (ATO-DLO), P.O. Box 17 6700 AA, Wageningen, the Netherlands |
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| Abstract: | Summary The accuracy and reproducibility of the gas-chromatographic method for the analysis of PHB and PHA in whole cells of Alcaligenes eutrophus H16 and Pseudomonas putida KT2442 were determined. It was found that for analysis of PHA the methanolysis time in the assay had to be increased to 4 h. Accuracy of the PHB and PHA assay were 0.018 mg and 0.304 mg respectively, based on estimation of the measurement error. |
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