Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China |
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Authors: | Huili Zhu Jinfeng Zhao Rongjun Wang Longxian Zhang |
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Affiliation: | The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China. |
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Abstract: | Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan. |
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