Clonal propagation of kurrat (Allium ampeloprasum var. kurrat) |
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Authors: | Yasseen Mohamed-Yasseen Suzanne Helene Costanza |
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Institution: | (1) IFAS, Tropical Research & Education Center, University of Florida, 33031 Homestead, Florida;(2) Notre Dame College of St. John's University, 300 Howard Avenue, 10304 Staten Island, New York |
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Abstract: | Summary Two protocols for clonal propagation of kurrat (Allium ampeloprasum var.kurrat) using explants from the basal plates of mature plants are described. In direct formation, explants were cultured in Murashige
and Skoog (MS) medium and supplemented with either benzyladenine at 0.0 or 4.4 μM, or supplemented with 7.0 μM benzyladenine and 0.1 μM naphthaleneacetic acid. Shoots appeared after 4 wk of culture. In the two-step procedure, explants were cultured first on
MS medium supplemented with 1.4 μM 2,4-dichlorophenoxyacetic acid and 1.4 μM kinetin, and incubated in the dark for 4 wk. They were then transferred to MS medium supplemented with 4.4 μM benzyladenine for shoot formation. All shoots were rooted on MS medium containing 5 g·liter−1 activated charcoal. Normal viable plants were successfully established in soil. |
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Keywords: | Allium kurrat stem base Egyptian Leek basal plate propagation |
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