Gibberellin biosynthetic pathway and the physiologically active gibberellin in the shoot ofCucumis sativus L. |
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| Authors: | Masayoshi Nakayama Hisakazu Yamane Noboru Murofushi Nobutaka Takahashi Lewis N Mander and Hideharu Seto |
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| Institution: | (1) Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Research School of Chemistry, The Australian National University, GPO Box 4, 2601 Canberra, ACT, Australia;(3) Present address: Department of Agricultural Chemistry, Utsunomiya University, 350 Mine-machi, 321 Utsunomiya, Japan;(4) Present address: The Institute of Physical and Chemical Research, 351-01 Wako-shi, Saitama, Japan |
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| Abstract: | 2H2]Gibberellin A24 (GA24) and 2H4]-GA9 were applied to the apices of normal-type cucumber (Cucumis sativus L. cv. Yomaki) seedlings treated with uniconazole, an inhibitor of GA biosynthesis. The metabolites from these feeds were identified by full-scan gas chromatography-mass spectrometry (GC-MS) to confirm the conversions of 2H2]GA24 to 2H2]GA9 and of 2H4]GA9 to 2H4]GA4. The results show that GA4 is biosynthesized from GA24
via GA9. In a cucumber hypocotyl elongation bioassay using cv. Yomaki, prohexadione (DOCHC), an inhibitor of 2-oxoglutaratedependent dioxygenase, inhibited the hypocotyl elongation caused by application of GA9, while DOCHC enhanced the elongation caused by application of GA4. These results indicate that GA4 is a physiologically active GA and that the activity of GA9 is due to its conversion to GA4 in cucumber shoots. |
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