Optimization of lipase production by <Emphasis Type="Italic">Staphylococcus warneri</Emphasis> EX17 using the polydimethylsiloxanes artificial oxygen carriers |
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Authors: | Fernanda Roberta Rech Giandra Volpato Marco Antônio Záchia Ayub |
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Institution: | (1) Food Science & Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Goncalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil;(2) Institute of Education, Science & Technology of Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, 2777, Brazil; |
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Abstract: | In this research, the combined effects of polydimethylsiloxane (PDMS) and different conditions of oxygen volumetric mass transfer
coefficient (kLa) on lipase production by Staphylococcus warneri EX17 were studied and optimized in bioreactor cultures. Raw glycerol from biodiesel synthesis was used as the sole carbon
source. Full-factorial central composite design and the response surface methodology were employed for the experimental design
and analysis of the results. The optimal polydimethylsiloxane concentration and mass coefficient transfer (kLa) were found to be 13.5% (v/v) and 181 h−1, respectively. Under these conditions, the maximal cell production obtained was 10.0 g/l, and the volumetric lipase activities
of approximately 490 U/l, after 6 h of cultivation. These results are in close agreement with the model predictions. Results
obtained in this work reveal the positive effects of PDMS on oxygen volumetric mass transfer coefficient (kLa) in the Staphylococcus warneri EX17 cultivation and lipase production. |
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