The stereospecific biosynthesis of plant sterols and α- and β-amyrin |
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Authors: | H H Rees E I Mercer and T W Goodwin |
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Institution: | Department of Biochemistry and Agricultural Biochemistry, University College of Wales, Aberystwyth |
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Abstract: | 1. A preparation of pea seedlings has been obtained that will incorporate 2-(14)C]mevalonate into squalene, alpha- and beta-amyrin and the phytosterols. 2. The (14)C/(3)H ratio in alpha- and beta-amyrin biosynthesized in the presence of 2-(14)C,4R-(3)H]-mevalonate is the same as in the starting material and in squalene; this gives experimental support to the mechanism for the cyclization of squalene proposed by Ruzicka for the formation of these pentacyclic triterpenoids. 3. The (14)C/(3)H ratio for beta-sitosterol was 5:3, the same as that in cholesterol in liver. 4. As the absence of (3)H from C-3 in beta-sitosterol was demonstrated (3)H must be present in the side chain and thus the H at C-24 is not lost during alkylation of the side chain; it probably migrates to C-25. |
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