| 一株人抗人A—血型物质单克隆抗体特异性的研究 |
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| 引用本文： | 陈华堂,Larrick J,Kabat E.A. 一株人抗人A—血型物质单克隆抗体特异性的研究[J]. 中国生物化学与分子生物学报, 1991, 7(1): 110-116 |
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| 作者姓名： | 陈华堂 Larrick J Kabat E.A |
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| 作者单位： | 安徽医科大学生化教研室，Cetus lmmune Corporation Palo Alto CA94303，Departments of Microbiology Genetics and Development and Neurology，College of Physicians and Snrgeona Columbia University New Yolk N. Y. 10032. U. S. A. 合肥 230022 |
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| 基金项目： | 国家教委《优秀年轻教师基金》 |
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| 摘 要： | 本文对一株人抗人A-血型物质单克隆抗体,用定量免疫沉淀法以及ELISA研究其与多种单糖、双糖及寡糖的反应性,从而确定了其结合部位的结构特异性。实验发现其结合部位互补于含有双分子岩藻糖残基的A-t糖:这一研究进一步强调了含有双分子岩藻糖残基的A血型抗原决定簇的重要性。
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| 关 键 词： | 单克隆抗体 结合部位 抗原决定基 |
| 收稿时间： | 1991-02-20 |
Studies on the specificize of a Human Monoclonal Antibody Against A Blood Group Substance |
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| Chen,Hua-Tang J.Larrick,E.A.Rabat,. Studies on the specificize of a Human Monoclonal Antibody Against A Blood Group Substance[J]. Chinese Journal of Biochemistry and Molecular Biology, 1991, 7(1): 110-116 |
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| Authors: | Chen Hua-Tang J.Larrick E.A.Rabat |
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| Affiliation: | (Department of Biochemistry, Anhui Medical University, Hefei, 230032Cetus Immune Corporatiin, Palo Alto, C.494303;Departments of Microbiobiology, Genetics and Development, and Neurology, College of Physicians and Surgeons, Columbia Uniersity, New York, New York 10032, U.S.A. |
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| Abstract: | This is the first human monoclnal antibody against A blood group substance, generated by Epstein-Barr virus trans-formation of huma lymphocyte isolated from splenic tissue after activation in vitro with group A blood cells.the antibody has been characterized by quantitative precipitin assays and the fins structure of its combining site has been mapped by quantitative ELISA assays by using A-active oligosaccharides to inhibit the binding of anti-A and A blood group substance. The human monoclonal anti-A strongly precipitated various A-active blood group substances, but not B, H, Lea, and the precusor of Ii substances.The combining site was most complemntary to the difucose containing A-Hep tasaccharide:GalNAcα1→3Galβ1→3GlcNAcβ1→3Galβ1→4Glc Fuca FucaThe se studies futher emphasize the importance of difucosyl determinants. |
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| Keywords: | Monoclonal antibody Combining site Antigenic determinant |
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