| 幽门螺杆菌毒素蛋白CagA诱导的蛋白的差异表达分析及其基因在胃癌组织中的表达 |
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| 引用本文： | 陈娴,周建奖,谢渊,赵艳,徐文杰,王妍. 幽门螺杆菌毒素蛋白CagA诱导的蛋白的差异表达分析及其基因在胃癌组织中的表达[J]. 中国生物化学与分子生物学报, 2011, 27(12): 1124-1131 |
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| 作者姓名： | 陈娴 周建奖 谢渊 赵艳 徐文杰 王妍 |
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| 作者单位： | 贵阳医学院分子生物学重点实验室;贵阳医学院附属医院检验系; |
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| 基金项目： | 国家自然科学基金资助项目(No.31060122); 贵州省科技攻关计划项目(No.黔科合SY字[2011]3067号)~~ |
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| 摘 要： | 为了研究胃癌细胞中幽门螺杆菌(Hp)毒素蛋白CagA诱导的蛋白差异表达及其基因在人胃癌组织中的表达,用Hp感染胃癌细胞系SGC 7901和AGS及用含CagA基因的表达载体稳定转染SGC 7901细胞, 构建3组实验模型.提取各组细胞的总蛋白进行双向凝胶电泳,筛选3组重叠的差异表达蛋白质斑点进行质谱鉴定.共获得135个差异表达的蛋白质,其中上调蛋白质73个,下调蛋白质62个. 鉴定出10个差异表达蛋白质, 其中有6个差异表达蛋白是首次发现，它们主要参与细胞的能量代谢和信号转导等.最后定量检测了这10个差异表达蛋白基因在人胃癌组织中的表达, 发现有4个基因高表达和1个基因低表达. 本结果将为研究幽门螺杆菌感染引起胃癌的分子机制提供新的线索.
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| 关 键 词： | 幽门螺杆菌 蛋白差异表达 胃癌 蛋白质组学 |
| 收稿时间： | 2011-08-29 |
Analysis of Differentially Expressed Proteins Induced by CagA in Gastric Cancer Cells and Their Expression in Gastric Cancers |
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| CHEN Xian),ZHOU Jian-Jiang),),XIE Yuan),ZHAO Yan),XU Wen-Jie),WANG Yan) Key Laboratory of Molecular Biology,Guiyang Medical University,Guiyang ,China,) Affiliated Hospital to Guiyang Medical University,China). Analysis of Differentially Expressed Proteins Induced by CagA in Gastric Cancer Cells and Their Expression in Gastric Cancers[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(12): 1124-1131 |
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| Authors: | CHEN Xian) ZHOU Jian-Jiang) ) XIE Yuan) ZHAO Yan) XU Wen-Jie) WANG Yan) Key Laboratory of Molecular Biology Guiyang Medical University Guiyang China ) Affiliated Hospital to Guiyang Medical University China) |
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| Affiliation: | CHEN Xian1),ZHOU Jian-Jiang1),2),XIE Yuan1),ZHAO Yan1),XU Wen-Jie1),WANG Yan1) (1) Key Laboratory of Molecular Biology,Guiyang Medical University,Guiyang 550004,China,2) Affiliated Hospital to Guiyang Medical University,China) |
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| Abstract: | To study the differential expression of the proteins induced by CagA in gastric cancer cells and their gene expression in human gastric cancer tissues,human gastric cancer cell lines SGC-7901 and AGS cells were infected with Helicobacter pylori(Hp).SGC-7901 cells were stably transfected with the vector pcDNA3.1ZEO(-) /CagA,carrying the full-length CagA gene,to establish three experimental models.Total proteins were extracted and then loaded on two-dimensional electrophoresis(2-DE).The differently expressed ... |
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| Keywords: | Helicobacter pylori differential expression of the proteins gastric carcinoma proteomics |
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