| 大黄素对金黄色葡萄球菌的抑菌作用机制 |
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| 引用本文： | 周磊,云宝仪,汪业菊,谢明杰. 大黄素对金黄色葡萄球菌的抑菌作用机制[J]. 中国生物化学与分子生物学报, 2011, 27(12): 1155-1159 |
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| 作者姓名： | 周磊 云宝仪 汪业菊 谢明杰 |
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| 作者单位： | 辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室; |
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| 基金项目： | 辽宁省教育厅项目(No.L2010236); 大连市计划项目(No.2009E12SF165)~~ |
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| 摘 要： | 以金黄色葡萄球菌为供试菌，通过测定大黄素对其细胞膜的通透性、可溶性蛋白质和呼吸代谢的影响，来阐述大黄素的抑菌作用机制. 利用电导率、生物大分子分析、呼吸代谢抑制检测等方法，验证大黄素的药效作用. 实验结果显示，大黄素作用金黄色葡萄球菌后，培养基溶液中电导率比对照组增加了2.23%，DNA和RNA大分子的含量比对照组增加了67.36%，大黄素作用金黄色葡萄球菌16 h后，菌体可溶性蛋白总量比对照组减少了28.3%；大黄素能抑制金黄色葡萄球菌物质代谢中的2种关键酶的活性，其中琥珀酸脱氢酶活性抑制率为53.8%，苹果酸脱氢酶的活性抑制率为25.5%.上述结果表明，大黄素可以破坏细菌细胞膜的通透性，抑制菌体内的蛋白质合成，通过抑制代谢关键酶的活性发挥杀菌作用.
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| 关 键 词： | 大黄素 金黄色葡萄球菌 抑菌机制 |
| 收稿时间： | 2011-09-19 |
Antibacterial Mechanism of Emodin on Staphylococcus aureus |
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| ZHOU Lei,YUN Bao-Yi,WANG Ye-Ju,XIE Ming-Jie. Antibacterial Mechanism of Emodin on Staphylococcus aureus[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(12): 1155-1159 |
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| Authors: | ZHOU Lei YUN Bao-Yi WANG Ye-Ju XIE Ming-Jie |
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| Affiliation: | ZHOU Lei,YUN Bao-Yi,WANG Ye-Ju,XIE Ming-Jie(College of Life Sciences,Liaoning Normal University,Key Laboratory of Biotechnology and Drug of Liaoning Province,Dalian 116029,Liaoning,China) |
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| Abstract: | Emodin is an effective bactericide.The antibacterial mechanism was carried out by determining membrane penetrability,the change of protein synthesis on Staphylococcus aureus,and the change of oxidative respiratory metabolism on bacteria.The results showed that electrical conductivity was increased by 2.23% with emodin than that in the control.The number of large molecules was increased by 67.36% than that in the control.After treated with emodin for 16 hours,the total content of proteins was decreased by 28... |
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| Keywords: | emodin Staphylococcus aureus antibacterial mechanism |
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