The T cell activation marker CD150 can be used to identify alloantigen-activated CD4(+)25+ regulatory T cells |
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Authors: | Browning Meghen B Woodliff Jeffrey E Konkol Marja C Pati Nirupma T Ghosh Soumitra Truitt Robert L Johnson Bryon D |
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Institution: | Department of Pediatrics, The Medical College of Wisconsin and the Children's Research Institute of the Children's Hospital of Wisconsin, Milwaukee, WI 53226, USA. mbrownin@mcw.edu |
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Abstract: | We have been investigating whether alloantigen-specific CD4(+)25+ regulatory T cells can be identified for use in treating graft-versus-host disease. CD150, which is upregulated on the surface of all activated T lymphocytes, was identified as a candidate marker for alloantigen-activated CD4(+)25+ regulatory T cells by gene chip analysis. Freshly isolated CD4(+)25+ cells had only low cell-surface expression of CD150, comparable to that of CD4(+)25- T cells. Increased CD150 expression was observed on all T cells after coculture with allogeneic stimulator cells. When purified CD4(+)25+ cells were precultured with allogeneic stimulator cells, then sorted into CD150+ and CD150- subsets, allosuppressive activity was contained primarily in the CD150+ fraction. These cells also suppressed the proliferation of alloantigen-activated autologous T cells, and they could be expanded in vitro without loss of their suppressive capacity. These results suggest that CD150 can be used as a marker for the identification of purified alloantigen-activated CD4(+)25+ regulatory T cells. |
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Keywords: | CD4+25+ cells CD150 Human regulatory T cells Graft vs host disease |
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