| 用cDNA微阵列研究内毒素休克小鼠肺组织基因表达谱的改变 |
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| 引用本文： | 袁开宇,赵震宇,刘瑛,邹江,刘梅冬,陈广文,尢家.用cDNA微阵列研究内毒素休克小鼠肺组织基因表达谱的改变[J].生物化学与生物物理进展,2002,29(3):402-406. |
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| 作者姓名： | 袁开宇 赵震宇 刘瑛 邹江 刘梅冬 陈广文 尢家 |
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| 作者单位： | 中南大学湘雅医学院病理生理学教研室,长沙,410078 |
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| 摘 要： | 利用cDNA微阵列检测了小鼠内毒素休克2 h及20 h肺组织基因表达谱的改变.发现内毒素休克2 h有128个基因表达上调，3个基因下调；内毒素休克20 h有51个基因表达上调，21个下调.并用RT-PCR进一步验证了结果的可靠性.初步分析了基因表达谱改变的意义.有助于从基因组水平阐明内毒素休克的分子机制.
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| 关 键 词： | 基因表达谱，内毒素休克，肺，小鼠 |
| 收稿时间： | 2001/9/11 0:00:00 |
| 修稿时间： | 2001年9月11日 |
A Study on The Changes of Gene Expression Profiles in Lung Tissues During Endotoxic Shock Using cDNA Miroarray |
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| YUAN Kai-Yu,ZHAO Zhen-Yu,LIU Ying,ZOU Jiang,LIU Mei-Dong,CHEN Guang-Wen,YOU Jia-Lu and XIAO Xian-Zhong.A Study on The Changes of Gene Expression Profiles in Lung Tissues During Endotoxic Shock Using cDNA Miroarray[J].Progress In Biochemistry and Biophysics,2002,29(3):402-406. |
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| Authors: | YUAN Kai-Yu ZHAO Zhen-Yu LIU Ying ZOU Jiang LIU Mei-Dong CHEN Guang-Wen YOU Jia-Lu and XIAO Xian-Zhong |
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| Institution: | Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China;Department of Pathophyshiology, Xiangya Medical College, Central South University, Changsha 410078, China |
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| Abstract: | The gene expression profiles of 1 176 genes were analyzed using AtlasTM Mouse cDNA array 1.2 in Babl/c mice lung tissues during endotoxic shock. Results showed that the expression of 128 genes was up-regulated and 3 genes down-regulated at 2 hours after exposure to endotoxin; the expression of 51 genes was up-regulated and 21 genes down-regulated at 20 hours after exposure to endotoxin. The changes in gene expression were further confirmed by RT-PCR. The significance of above changes in gene expression was analyzed. The results would be helpful to elucidating the molecular mechanism of endotoxic shock. |
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| Keywords: | gene expression profiles endotoxin shock lungs mice |
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