The structural topology of wild-type phospholamban in oriented lipid bilayers using 15N solid-state NMR spectroscopy |
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Authors: | Abu-Baker Shadi Lu Jun-Xia Chu Shidong Shetty Kiran K Gor'kov Peter L Lorigan Gary A |
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Affiliation: | Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA. |
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Abstract: | For the first time, 15N solid-state NMR experiments were conducted on wild-type phospholamban (WT-PLB) embedded inside mechanically oriented phospholipid bilayers to investigate the topology of its cytoplasmic and transmembrane domains. 15N solid-state NMR spectra of site-specific 15N-labeled WT-PLB indicate that the transmembrane domain has a tilt angle of 13 degrees+/-6 degrees with respect to the POPC (1-palmitoyl-2-oleoyl-sn-glycero-phosphocholine) bilayer normal and that the cytoplasmic domain of WT-PLB lies on the surface of the phospholipid bilayers. Comparable results were obtained from site-specific 15N-labeled WT-PLB embedded inside DOPC/DOPE (1,2-dioleoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) mechanically oriented phospholipids' bilayers. The new NMR data support a pinwheel geometry of WT-PLB, but disagree with a bellflower structure in micelles, and indicate that the orientation of the cytoplasmic domain of the WT-PLB is similar to that reported for the monomeric AFA-PLB mutant. |
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Keywords: | wild-type phospholamban oriented phospholipids structural topology 15N solid-state NMR spectroscopy |
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