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Forming of a functional biofilm on wood surfaces
Authors:Michael F. Sailer  Elke J. van Nieuwenhuijzen  Wieger Knol
Affiliation:1. Built Environment and Geosciences, Organization for Applied Scientific Research, van Mourik Broekmanweg 6, 2600 AA Delft, Netherlands;2. Quality of Life, Organization for Applied Scientific Research, Utrechtseweg 48, 3704 HE Zeist, Netherlands;1. Faculdade de Ciências Agrárias e Veterinárias, UNESP - Universidade Estadual Paulista, “Julio de Mesquita Filho”. Postgraduate in Agricultural Microbiology, CEP 14884-900, Jaboticabal, SP, Brazil;2. Centro de Citricultura “Sylvio Moreira”/IAC, CEP 13490-970, Cordeirópolis, SP, Brazil;1. CBS KNAW Fungal Biodiversity Centre, Applied and Industrial Mycology, Uppsalalaan 8, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;2. Saxion, University of Applied Sciences, Centre for Urban and Environmental Development, Tromplaan 28, P.O. Box 70.000, 7500 KB Enschede, The Netherlands;3. Regge Hout B.V., Breukersweg 9, 7471 ST Goor, The Netherlands;4. NIBIO, Norwegian Institute of Bioeconomy Research, P.O. Box 115, NO-1431 Ås, Norway;5. Eindhoven University of Technology, Department of Applied Physics, Den Dolech 2, P.O. Box 513, 5600 MB Eindhoven, The Netherlands;6. TNO, Microbiology & Systems Biology, Utrechtseweg 48, P.O. Box 360, 3700 AJ Zeist, The Netherlands;1. Leibniz University Hannover, Faculty of Architecture and Landscape Scape Sciences, Institute of Vocational Sciences in the Building Trade, Hannover, Germany;2. Lund University, Faculty of Engineering, Division of Structural Engineering, Lund, Sweden;1. Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA;2. Biochemistry Unit, Department of Medical Sciences, Faculty of Science, Rangsit University, Patumthani 12000, Thailand
Abstract:The protecting and staining properties of biofilms grown on oil-treated surfaces of Pinus sylvestris L. sapwood were investigated with respect to their potential to create homogeneous coloured surfaces. Linseed oil pressure-treated blocks of P. sylvestris L. were evaluated after 36 months of outdoor exposure. The biofilm was characterized by colony counts and PCR cloning, the interactions with wood were assessed microscopically. The results show that a biofilm consisting of Aureobasidium pullulans has the potential to create protecting and staining functions on a wood surface. The conditions and factors which lead to a selective growth of A. pullulans are discussed with respect to the practical application of the formed biofilm in the field of environmental and civil engineering.
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