四川王朗自然保护区大熊猫主食竹——缺苞箭竹(Fargesia denudat)不同发育时期酯酶和α-淀粉酶同工酶的研究

通过聚丙烯酰胺凝胶电泳,对生长在亚高山地带的大熊猫主食竹的实生苗、成竹和开花竹中不同发生年龄鞭系,以及同一鞭系内一系列生长年龄竹株作了酯酶、α-淀粉酶同工酶的比较研究。结果表明,同一竹种的不同发生年龄鞭系之间叶酯酶电泳类型存在明显差异,而同一鞭系内α-淀粉酶同工酶的变化表现与竹株生长年龄有关。由此认为,上述同工酶系统可以用作竹类无性系发育分析的生理指标。

STUDIES ON ESTERASE AND α-AMYLASE ISOZYMES AT DIFFERENT DEVELOPMENT STAGE OF FARGESIA DENUDAT——A MAIN EDIBLE BAMBOO FOR GIANT PANDAS IN THE NATURAL PROTECTED AREA WANGLANG,SICHUAN PROVINCE

The comparetive studies of esterase and α-amylase isozymes on the bamhoo rhizome systems with different developmental ages, and standing culms in same rhizome with a series of growthages were made by means of polyacrylamide gel electrophoresis. The samples were from edible bamboo species for giant pandas in sub-mountain areas, including seedlings, full-grown and flowering bamboo plants. The results showed that the leaf EST can present various electrophoretic patterns for different rhizome systems of the same species. But the Shifts in AMY isozymes appear related to age level of bamboo culms. As a consequence it is suggested that two of above mentioned isozyme systems may be used as a physiological index for development analysis of bamboo clone.