Regulation of rDNA stability by sumoylation |
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| Authors: | Nadine Eckert-Boulet Michael Lisby |
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| Affiliation: | 1. Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland;2. Biosystems Group, Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland;1. Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK;2. College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK;1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 8 Lavrentyev Ave, Novosibirsk 630090, Russian Federation;2. Department of Natural Sciences, Novosibirsk State University, 2 Pirogova St, Novosibirsk 630090, Russian Federation;3. Groupe «Réparation de l''ADN», Equipe Labellisée par la Ligue Nationale Contre le Cancer, CNRS UMR8200, Univ. Paris-Sud, Université Paris-Saclay, F-94805 Villejuif, France;4. Gustave Roussy, Université Paris-Saclay, F-94805 Villejuif, France;1. The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China;2. Institute for Systems Biology, Seattle, WA 98109, United States;1. Institute of Human Genetics, UPR 1142, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France;2. Equipe Labellisée Ligue Contre le Cancer, 14 rue Corvisart, 75013 Paris, France;3. Department of Medical Biochemistry and Biophysics, Umeå University, SE-901 87 Umeå Sweden;4. Inserm U847, Centre Hospitalier Universitaire de Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint Eloi, Université Montpellier 1, Montpellier F-34000, France;5. MGX-Montpellier GenomiX, c/o Institut de Génomique Fonctionnelle, 141 rue de la Cardonille, F-34094 Montpellier Cedex 5, France;6. Laboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland;7. Department of Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan |
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| Abstract: | Repair of DNA lesions by homologous recombination relies on the copying of genetic information from an intact homologous sequence. However, many eukaryotic genomes contain repetitive sequences such as the ribosomal gene locus (rDNA), which poses a risk for illegitimate recombination. Therefore, the eukaryotic cell has evolved mechanisms to favor equal sister chromatid exchange (SCE) and suppress unequal SCE, single-strand annealing and break-induced replication. In the budding yeast Saccharomyces cerevisiae, the tight regulation of homologous recombination at the rDNA locus is dependent on the Smc5–Smc6 complex and sumoylation of Rad52, which directs DNA double-strand breaks in the rDNA to relocalize from within the nucleolus to the nucleoplasm before association with the recombination machinery. The relocalization before repair is important for maintaining rDNA stability. The focus of this review is the regulation of recombinational DNA repair at the rDNA locus by sumoylation and the Smc5–Smc6 complex in S. cerevisiae. |
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